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ZoP Cytotoxic Activity of Calabash (Crescentia cujete) Leaf Crude Extract Against Human Breast Cancer Cell Line (MCF7) and Chinese Hamster Ovarian Normal Cell Line (AA8)

autorzy: Kyla Loisse Isabelle N. Gascon Cheval L. Bahalla Claire L. Villarmia Erika dP. Ondap Chris Jasmine T. Niog

This study aimed to verify whether Calabash leaf (Crescentia cujete) is a potential anti-breast cancer remedy. Five hundred grams of air dried Calabash leaf was placed in a container and soaked with ethanol for 72 hours. Then it was filtered and the filtrate underwent Rotavap to get the crude extract. The extract was tested for its cytotoxic activity against the human breast cancer cell (MCF7) and Chinese hamster ovarian normal cell (AA8). Results showed that Crescentia cujete leaf crude extract has an inhibitory concentration 50 (IC50) of 8.7267 µg/mL which means that it has an anti-proliferation activity against human breast cancer cell ( MCF7). Crescentia cujete leaf extract also showed that it is slightly toxic to normal cell (AA8) since it has an IC50 of 17.5633 µg/mL. The extract however is more toxic in cancer cell than in normal cell. The positive control doxorubicin is found to be highly toxic in cancer cell and normal cell showing an IC50 of 2.373 ug/mL and 1.73ug/mL for cancer cell (MCF7) and normal cell (AA8) respectively. Thus, Calabash leaf could be a potential source of breast cancer treatment.